Reporter

Part:BBa_K3026014:Design

Designed by: Pascale Lemieux   Group: iGEM19_ULaval   (2019-10-16)


Toehold measles virus MG912594.1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 167


Design Notes

ToeHold were designed with sequence constraints according to the engineered design from Green et al. 2014. sfGFP was chosen according to its expression profile in cell-free media containing T7 polymerase, and according to its excitation and emission spectra.


Source

Synthetic construct. Switch sequence comes from the reference genome of measles virus genotype H1 but is also conserved in genotypes C2, B3, G2, D8, and D4. sfGFP sequence comes from part BBa_K1321337. Other ToeHold sequence come from Green et all 2014 Supp. Data switch #1

References